TPH Wizard

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Step 1: Calibration

Step 2

Step 3

Step 4

Step 5

Step 1 — Calibration Setup

Review retention times from the .gcm method file and confirm the RT window for each calibration compound. The wizard will then integrate peaks from the Standard GCD files to build calibration curves.

Batch: 20260113 solid - Total Petroleum Hydrocarbon - TPH · 6 Standard file(s)

C10-C14 as C14 — RT 16.0236 min Levels: 2.51, 5.01, 10.02, 25.05, 50.10 ppm

RT Window (min)

Band

r² = 0.9985

C15-C28 as C26 — RT 33.1488 min Levels: 2.51, 5.01, 10.03, 25.08, 50.15 ppm

RT Window (min)

Band

r² = 0.9996

C29-C36 as C32 — RT 49.1112 min Levels: 2.51, 5.01, 10.02, 25.05, 50.10 ppm

RT Window (min)

Band

r² = 0.9998

C42 — RT 58.5919 min Levels: 2.41, 4.83, 9.66, 24.14, 48.29 ppm

RT Window (min)

Band

r² = 0.9986

Standard File Chromatograms — MOCCA2 Integration

Click the zoom button on any chromatogram to enlarge it. Detected peaks are coloured and labelled. Adjust MOCCA2 parameters and re-run to refine peak detection.

MOCCA2 Parameters

(applied to all standards)

RT Start

RT End

Baseline

Smoothness

p (asym.)

Min Height %

Min Rel H

Split

Merge

Min Width

Max Width

Width At

Expand

QC_STD 1 _007.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 1 _007.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

QC_STD 2_008.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 2_008.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

QC_STD 3_009.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 3_009.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

QC_STD 4_010.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 4_010.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

QC_STD 5 primer_006.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 5 primer_006.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

QC_STD 5_011.gcd

Standard view raw 0 peak(s) detected

X: Min Max Y: Min Max or drag on image

Chromatogram for QC_STD 5_011.gcd

Drag left→right on image to zoom · Hover to see coordinates · Enter x-min/x-max then press Apply

⚠ No peaks detected with current parameters. Try widening the RT window or lowering detection thresholds.

Compound	Band	Levels (ppm)	Slope	Intercept	r²	Status
C10-C14 as C14		2.505000114440918, 5.010000228881836, 10.020000457763672, 25.049999237060547, 50.099998474121094	11976.54	17892.55	0.9985	✓ Pass
C15-C28 as C26		2.507999897003174, 5.014999866485596, 10.029999732971191, 25.075000762939453, 50.150001525878906	10897.36	5919.39	0.9996	✓ Pass
C29-C36 as C32		2.505000114440918, 5.010000228881836, 10.020000457763672, 25.049999237060547, 50.099998474121094	10678.13	-2485.26	0.9998	✓ Pass
C42		2.4140000343322754, 4.828999996185303, 9.656999588012695, 24.14299964904785, 48.2859992980957	9961.03	-7095.38	0.9986	✓ Pass

r² ≥ 0.99 required per SOP. If any curve fails, adjust RT windows or flag for manual review.